A non-disruptive three-dimensional culture system allows cell growth and proliferation
in three dimensions, permitting cell splitting without subjecting cells to disruptive
conditions that affect cell structure and functions. An extracellular matrix provides
a good environment for culturing or co-culturing anchorage-dependent cells. The
cells cultured this manner can be readily used in such applications as cell transplantation,
tissue engineering seeding of cells on scaffolds, and other applications that require
immediate availability of functioning cells.