This invention provides prokaryotic glycosyltransferases, including a bifunctional
sialyltransferase that has both an 2,3- and an 2,8-activity. A 1,4-GalNAc
transferase and a 1,3-galactosyltransferase are also provided by the invention,
as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide
(LOS). The glycosyltransferases can be obtained from, for example, Campylobacter
species, including C. jejuni. In additional embodiments, the invention
provides nucleic acids that encode the glycosyltransferases, as well as expression
vectors and host cells for expressing the glycosyltransferases.
