This invention provides for compositions for use in real time nucleic acid
detection processes. Such real time nucleic acid detection processes are
carried out with energy transfer elements attached to nucleic acid
primers, nucleotides, nucleic acid probes or nucleic acid binding agents.
Real time nucleic acid detection allows for the qualitative or
quantitative detection or determination of single-stranded or
double-stranded nucleic acids of interest in a sample. Other processes
are provided by this invention including processes for removing a portion
of a homopolymeric sequence, e.g., poly A sequence or tail, from an
analyte or library of analytes. Compositions useful in carrying out such
removal processes are also described and provided.
