A method for multiplexed detection and quantification of analytes by reacting
them
with probe molecules attached to specific and identifiable carriers. These carriers
can be of different size, shape, color, and composition. Different probe molecules
are attached to different types of carriers prior to analysis. After the reaction
takes place, the carriers can be automatically analyzed. This invention obviates
cumbersome instruments used for the deposition of probe molecules in geometrically
defined arrays. In the present invention, the analytes are identified by their
association with the defined carrier, and not (or not only) by their position.
Moreover, the use of carriers provides a more homogenous and reproducible representation
for probe molecules and reaction products than two-dimensional imprinted arrays
or DNA chips.
