The present invention provides porcine isogloboside 3 (iGb3) synthase
protein, cDNA, and genomic DNA regulatory sequence. The present also
invention includes porcine animals, tissue and organs as well as cells
and cell lines derived from such animals, tissue and organs, which lack
expression of functional iGb3 synthase. Such animals, tissues, organs and
cells can be used in research and in medical therapy, including
xenotransplantation. In addition, methods are provided to prepare organs,
tissues, and cells lacking the porcine iGb.sub.3 synthase gene for use in
xenotransplantation.
