Red blood cells can be loaded with low molecular weight nitrosylating agents,
such as S-nitrosothiols, to act as a delivery system for NO+ groups
to tissues. Loaded red blood cells can be used in methods of therapy for conditions
which are characterized by abnormal O2 metabolism of tissues, oxygen-related
toxicity, abnormal vascular tone, abnormal red blood cell adhesion, or abnormal
O2 delivery by red blood cells. Such treatment of red blood cells can
be extended to in vivo therapies, with the object to achieve an increase in the
ratio of red blood cell S-nitrosothiol to hemoglobin.
