The invention describes systems and methods for calibrating a low-level light
imaging system. Techniques described herein employ a light calibration device that
is placed within a low level light imaging box to calibrate the system and its
constituent components such as the camera and processing system. The calibration
device comprises an array of low-power light supplies each having a known emission.
By taking an image of each low-power light supply, and comparing the processed
result with the known emission, the accuracy of the imaging system and its absolute
imaging characteristics may be assessed and verified.