Dibenzorhodamine compounds having the structure
##STR1##
are disclosed, including nitrogen- and aryl-substituted forms thereof. In addition,
two intermediates useful for synthesizing such compounds are disclosed, a first
intermediate having the structure
##STR2##
including nitrogen- and aryl-substituted forms thereof, and a second intermediate
having the structure
##STR3##
including nitrogen- and aryl-substituted forms thereof, wherein substituents
at positions C-14 to C18 taken separately are selected from the group consisting
of hydrogen, chlorine, fluorine, lower alkyl, carboxylic acid, sulfonic acid, -CH2OH,
alkoxy, phenoxy, linking group, and substituted forms thereof. The invention further
includes energy transfer dyes comprising the dibenzorhodamine compounds, nucleosides
labeled with the dibenzorhodamine compounds, and nucleic acid analysis methods
employing the dibenzorhodamine compounds.
