Generation of longer cDNA fragments from SAGE tags for gene identification
(GLGI) is disclosed. This method converts SAGE tags, which are about 10 base pairs
in length, into their corresponding 3 cDNA fragments covering hundred bases.
This added information provides for more accurate genome-wide analysis and overcomes
the inherent deficiencies of SAGE. The generation of longer cDNA fragments from
isolated and purified protein fragments for gene identification is also disclosed.
This method converts a short amino acid sequence into extended versions of the
DNA sequences encoding the protein/protein fragment and additional 3 end
sequences of the gene encoding the protein. This additional sequence information
allows gene identification from purified protein sequences. The invention also
provides a high-throughput GLGI procedure for identifying genes corresponding to
a set of unidentified SAGE tags.
