Raffinose is produced by allowing a raffinose synthase having the
following properties to act on sucrose and galactinol:
(1) action and substrate specificity: produces raffinose from sucrose and
galactinol;
(2) optimum pH: about 6 to 8;
(3) optimum temperature: about 35 to 40.degree. C.;
(4) molecular weight:
(i) about 75 kDa to 95 kDa estimated by gel filtration chromatography;
(ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel
electrophoresis (Native PAGE); and
(iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel
electrophoresis under a reduced condition (SDS-PAGE); and
(5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and
myo-inositol.
