Probe sets for the multiplexed detection of known, selected nucleotide target
sequences are provided. Detection involves the release of identifying tags as a
consequence of target recognition. The probe sets include electrophoretic tag probes
or "e-tag probes", comprising a detection region and a mobility-defining region
called the mobility modifier, both linked to a target-binding moiety. The target-binding
moiety of the e-tag probes hybridizes to complementary target sequences followed
by nuclease cleavage of the e-tag probes and release of detectable e-tags or e-tag
reporters. The mixture is exposed to a capture agent which binds uncleaved and/or
partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed
assay, different released e-tag reporters may be separated and detected providing
for target identification.
