Methods for the formation of sulfurized oligonucleotides are provided. The
methods allow for the formation of phosphorothioate linkages in the
oligonucleotides or derivatives, without the need for complex solvent
mixtures and repeated washing or solvent changes. Oligonucleotides having
from about 8, and up to about 50, nucleotides can be sulfurized according
to the methods of the invention with higher yields than have been
previously reported.
