Disclosed are methods of preparing high-quality polyunsaturated fatty
acid-containing lipids from a lipid-containing material that include
enzymatic treatment of components of the material and/or pressure
disruption of the material. Lipid-containing materials include biomass,
such as microorganisms. The invention further includes products
containing the lipid compositions, such as dietary supplements, food
products, pharmaceutical formulations, humanized animal milk, and infant
formula.