Methods for preparing nanoscale reactions using nucleic acids are presented.
Nucleic acids are captured saturably, yet reversibly, on the internal surface of
the reaction chamber, typically a capillary. Excess nucleic acid is removed and
the reaction is performed directly within the capillary. Alternatively, the saturably
bound nucleic acid is eluted, dispensing a metered amount of nucleic acid for subsequent
reaction in a separate chamber. Devices for effecting the methods of the invention
and a system designed advantageously to utilize the methods for high throughput
nucleic acid sequencing reactions are also provided.
