Pressure-driven microfluidic separation devices, such as may be used
for performing high performance liquid chromatography, are provided. Multiple separation
columns may be defined in a single device and packed with stationary phase material
retained by porous frits. One or more splitters may be provided to distribute slurry
and/or mobile phase among multiple separation columns. In one embodiment, separation
devices are substantially planar and fabricated with multiple device layers. Systems
and methods employing slurry for packing separation devices are also provided.
