A bioproduct may be selectively separated from one or more impurities by
means of a displacement chromatography system that includes a solvent, a
chromatographic resin and a chemically selective displacer. The method
includes:
dissolving the bioproduct and the one or more impurities in a solvent;
loading the bioproduct and the one or more impurities, in the solvent, on
a chromatographic resin;
displacing the bioproduct from the chromatographic resin with chemically
selective displacer; and
retaining the one or more impurities on the chromatographic resin.
For this method, the bioproduct and the impurities have similar binding
affinity for the chromatographic resin in the absence of the displacer.
