The invention provides genes from the I2 Fusarium resistance locus of tomato belonging to a multigene family herein designated I2C. The DNA molecules of the invention are useful as a tomato resistance gene to plant vascular diseases caused by Fusarium pathogens, particularly Fusarium oxysporium f.sp. lycopersici race 2, or as probes for breeding Fusarium-resistant tomato lines or for screening of news diseases in plants of the Solanaceae family. Further provided are Fusarium-resistant tomato lines transformed by an I2C resistance gene of the invention.