An apparatus for positioning and marking a location of an electromyography (EMG) electrode includes a housing having a back surface, an EMG sensor for receiving the EMG signal, an amplifying circuit for amplifying the EMG signal to generate an amplified EMG signal and an audio output terminal for outputting the amplified EMG signal as the audio EMG signal. The examinee may directly position and mark the location of the EMG electrode and may easily detect the fatigue rate of the muscle or the abnormal condition of the muscle by using the sound of the audio equipment.