The present invention provides nucleic acid and amino acid sequences of acetyl
CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase
(ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde
dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also
provides a recombinant vector comprising a nucleic acid sequence encoding one of
the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor,
a cell transformed with such a vector, antibodies to the enzymes, a plant cell,
a plant tissue, a plant organ or a plant in which the level of an enzyme has been
altered, and a method of producing such a plant cell, plant tissue, plant organ
or plant. Desirably, alteration of the level of enzyme results in an alteration
of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant.
In addition, the present invention provides a recombinant vector comprising an
antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC),
the E1 subunit of pPDH, the E1 subunit of pPDH, the E2 subunit of
pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH)
or a ribozyme that can cleave an RNA molecule encoding PDC, E1 pPDH, E1
pPDH, E2 pPDH, mtPDH or ALDH.