An instrument for conducting nucleic acid amplification reactions in a disposable
test device. The test device includes a first reaction chamber containing a first
nucleic acid amplification reagent (e.g., primers and nucleotides) and a second
reaction chamber either containing, or in fluid communication, with a second nucleic
acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument
includes a support structure receiving the test device. A temperature control system
maintains the first reaction chamber at a first elevated temperature but simultaneously
maintains the second nucleic acid amplification reagent at a second temperature
lower than the first temperature so as to preserve the second nucleic acid amplification
reagent. An actuator operates on a fluid conduit in the test device to place the
first and second reaction chambers in fluid communication with each other after
a reaction has occurred in the first reaction chamber at the first temperature.
A pneumatic system is also provided that assists in fluid transfer of a reaction
solution from the first chamber to the second chamber.