The present invention provides systems and methods for determining the
presence or absence of one or more target nucleic acid sequences in a
sample. Also provided are kits comprising these systems, and uses of
these systems in such applications as determining the presence or absence
of at least one target nucleic acid sequence in a sample, detecting
microorganism transcripts and host transcripts, differentiating
microorganism transcripts from host transcripts, screening blood
products, assaying a food product for contamination, assaying a sample
for environmental contamination, detecting genetically-modified
organisms, biodefense, forensics, and genetic-comparability studies. The
present invention further provides a complex that includes a target
nucleic acid sequence, a capture nucleic acid, and a reporter nucleic
acid.
