The present invention relates to a mutated pyruvate carboxylase gene from Corynebacterium.
The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which
is resistant to feedback inhibition from aspartic acid. The present invention also
relates to a method of replacing the wild-type pyruvate carboxylase gene in Corynebacterium
with this feedback-resistant pyruvate carboxylase gene. The present invention
further relates to methods of the production of amino acids, preferably lysine,
comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.