A method for isolating the bioactive component of the water-soluble extract of
Uncaria tomentosa known as C-MED-100, comprising (i) precipitating
the spray drying carrier from C-MED-100; (ii) using the resulting C-MED-100
to obtain a spotting mixture for thin layer chromatography (TLC); (iii) spotting
the C-MED-100 spotting mixture on pre-run TLC plates and eluting the plates
to obtain the fluorescing band with Rf=0.2-0.3; (iv) scraping off the
Rf=0.2-0.3 band, eluting it in ammonia and freeze drying the eluted
band to form a powder; and (v) extracting the powder with methanol to remove solubilized
silica gel, concentrating the methanol solution and crystalizing the concentrated
solution to obtain the bioactive component. The isolated bioactive component is
a quinic acid analog, preferably quinic acid lactone. A pharmaceutical composition
comprising a pharmaceutically effective amount of the bioactive component and a
nontoxic inert carrier or diluent. The bioactive component may be used to enhance
immune competency, treat disorders associated with the immune system, inhibit the
inflammatory response, treat disorders associated with the inflammatory response,
enhance the anti-tumor response, and treat disorders associated with the response
to tumor formation and growth, all in mammals.