The present invention includes recombinant proteins derived from Clostridium
botulinum toxins. In particular, soluble recombinant Clostridium botulinum
type A, type B and type E toxin proteins are provided. Methods which allow
for the isolation of recombinant proteins free of significant endotoxin contamination
are provided. The soluble, endotoxin-free recombinant proteins are used as immunogens
for the production of vaccines and antitoxins. These vaccines and antitoxins are
useful in the treatment of humans and other animals at risk of intoxication with
clostridial toxin.