A new chemical compound for treating human inflammatory diseases, tissue damage,
stroke, septic shock and cancer. This chemical compound is produced from a process
including the following steps: (a) washing human foreskin fibroblasts with a fresh
DMEM medium then incubated in a fresh medium containing 2.5%-10% fetal bovine serum
for at least 8 hours to form a proliferating phase medium (PPM); (b) subjecting
the proliferating phase medium to a 10-kDa ultrafiltration membrane to separate
the proliferating phase medium into a 10 kDa fraction, which passes through
the 10-kDa ultrafiltration membrane, and a 10 kDa fraction, which is retained
by the 10-kDa ultrafiltration membrane; (c) Chromatographing the 10 kDa fraction
on a Superdex 30 column by FPLC to separate the 10 kDa fraction into five
post-FPLC fractions; (d) Injecting the second post-FPLC fraction into a C18
column in a HPLC system with a gradient elute from 10% to 50% acetonitrile and
water containing 0.1% trifluoroacetic acid, to separate the second post-FPLC fraction
into five post-HPLC fractions; and (e) collecting the fourth post-FPLC fraction
for further purification. Fluorescence spectroscopy shows that this product is
a derivative of trypophan or trypophan-like, and mass spectrometry indicates that
it has a molecular weight of about 604 daltons with a minor peak having a molecular
weight of about 779 daltons.