A glycoside in which at least two phenolic hydroxyl groups of a compound having at least two phenolic hydroxyl groups in its molecule are glycosidically linked to anomeric carbon atoms of at least two sugar molecules can be prepared with high yield by a method including the step of allowing phenolic hydroxyl groups of a compound having at least two phenolic hydroxyl groups in its molecule to react with sugar molecules each having an acetylated hydroxyl group attached to an anomeric carbon atom in the presence of an organic solvent and an acid catalyst while removing acetic acid formed during the reaction from the reaction system.