We describe a food additive comprising a PS4 variant polypeptide, in which
the PS4 variant polypeptide is derivable from a parent polypeptide having
non-maltogenic exoamylase activity, in which the PS4 variant polypeptide
comprises an amino acid substitution at position 223 with reference to
the position numbering of a Pseudomonas saccharophilia exoamylase
sequence shown as SEQ ID NO: 1. The PS4 variant polypeptide may further
comprise one or more further mutations at a position selected from the
group consisting of: 121 and 161, preferably 121F, 121Y, 121W and 161A,
more preferably G121F, G121Y, G121W and/or S161A, or the group consisting
of: 134, 141, 157, 223, 307, 334, 33 and 34, preferably G134R, A141P,
I157L, G223A, H307L, S334P, N33Y and D34N. In preferred embodiments, the
PS4 variant polypeptide further comprises a mutation at position 87,
preferably G87S, a mutation at position 178, preferably L178F, and/or a
mutation at position 179, preferably A179T.