A purified vitamin B₆-phosphate phosphatase (VB6PP), having the following physico-chemical properties: a) Molecular weight: 29,0005,000 (consisting of a monomer having a molecular weight of 29,0005,000); b) Co-factor: Mn²⁺, Mg²⁺, Co²⁺, Sn²⁺, or Ni²⁺; c) Substrate specificity: active on pyridoxol 5-phosphate, pyridoxal 5-phosphate and pyridoxamine 5-phosphate; d) Optimum temperature: 30-400 C. at pH 7.5; and e) Optimum pH: 7.0-8.0 is disclosed. Also disclosed are a process for producing VB6PP from a microorganism belonging to the genus Sinorhizobium capable of producing VB6PP, a process for producing vitamin B₆ from vitamin B₆-phosphate (VB6P) utilizing VB6PP, and a cell-free extract of a microorganism belonging to the genus Sinorhizobium capable of producing VB6PP.