The present invention is a sample holder for spectrum measurement settable in a measuring chamber of a spectrophotometer and being used for measuring a spectrum of a liquid sample, which comprises a holding block (11) having a hole (111) and a light introducing path (112) being provided crosswise, and a light introducing path position adjusting means (12) being located under the holding block (11), said the light introducing path position adjusting means (12) comprising a light introducing path horizontal direction position adjusting means (12-1) and/or a light introducing path vertical direction position adjusting means (12-2), for adjusting so as to the irradiation light is introduced into the liquid sample by aligning a position of the light introducing path (112) with a propagating route of the irradiation light for spectrum measurement, said the holding block (11) further comprising a device for controlling a temperature of said liquid sample.