Expression of recombinant polysaccharide degrading enzymes in plants is
described. In one embodiment, expression of the enzyme is preferentially
directed to the seed of the plant. Expression may also be preferentially
targeted to specific locations within the plant cell. Expression of
cellulases in corn is shown. The result is the capacity to produce
polysaccharide degrading enzymes in plants at commercially acceptable
levels in a reliable manner. Methods of using same in production of
ethanol is also described, including use of the plant-produced enzymes in
the ethanol production process.