The present invention relates to a method for determining an effective
dose of monochromatic or polychromatic light from one or more light
sources to inactivate microorganisms present in a biological fluid,
preferably a non-transparent fluid. Moreover, there is provided a method
for the inactivation of microorganism in a biological fluid in a
flow-through-reactor. Moreover, the invention advantageously provides a
flow-through-reactor with one or more thermostated light sources. The
invention further provides a method of controlling the light sum dose of
monochromatic or polychromatic light emitted from one or more light
sources to effectively inactivate microorganisms present in a biological
fluid in a batch reactor.