A method for soft configuring communication protocols is applied to a memory device which is capable of communication by using multiple protocols. The memory device comprises a control unit for detecting the type of protocol supported by an external device connected therewith. The method performs a detecting sequence based on a configure file stored in the memory device, so as to set up a preferred prior order of protocols when the memory device is switched to use one of the protocols corresponding to the supported protocol of the external device. In addition, by changing either the status of a write protect button of the memory device, the file name of the configure file, or the content of the detecting sequence, the user can modify the preferred prior order of protocols, and thus achieves the function of soft configuring communication protocols.