A process is provided for inducing direct somatic embryogenesis in Pooideae and
rapidly regenerating fertile plants by first culturing isolated immature scutella
cells in culture medium comprising auxin, cytokinin and polyamine in amounts effective
to cause direct formation of primary embryos without an intervening callus stage,
at least until at least one primary embryo reaches the globular developmental stage,
the auxin being present in greater proportion than cytokinin. A second step includes
either a) culturing the primary embryos under conditions to regenerate plantlets,
and culturing the primary embryos in regeneration medium; or b) culturing the primary
embryos at the globular developmental stage and no longer than the coleoptilar
stage in culture medium comprising auxin, cytokinin, and polyamine in amounts effective
to cause induction of secondary embryo formation, at least until secondary embryogenesis
is detected, the cytokinin being present in greater proportion than auxin, and
culturing the secondary embryos under conditions to regenerate plantlets.