A toothbrush assembly includes a removable cartridge housing a spring biased piston dentifrice in a storage area. The cartridge is received in a canister attached to a brush head stem in which a passage is formed. The piston forces a quantity of the dentifrice through a supply tube in the cartridge to the passage that connects to an aperture in the brush head. A normally closed valve in the passage controls the flow of the dentifrice. The piston can be removed for refilling the cartridge with the dentifrice. Alternatively, the used cartridge may be replaced with a new prefilled disposable cartridge.