A method is described for controllably conducting complex PCR amplifications,
wherein
at least the following steps are conducted:
- a) PCR amplification with at least 50 primers of a first type (type
1) of different sequence, which are complementary to one of the strands of a random
DNA sample, and also with a primer or a library of primers of a second type (type
2), which is complementary to the other strand of the DNA sample used, wherein
the type 2 primers contain a first label (label 1);
- b) hybridizing of the amplified products to an oligomer array, which
comprises oligonucleotides that hybridize to the primers utilized in the PCR reaction
or to oligonucleotides that are complementary to these;
- or hybridizing of the amplified products to an oligomer array, which
contains oligomers complementary to the primers utilized in the PCR reaction;
- c) length determination of the amplified products bound to the array
by a second label (label 2) which can be correlated with the length of the respective
DNA fragment, and which is different from the first label (label 1) in step a) and
- d) quantification of the signals originating from label 1 and label
2 at each site of the oligonucleotide array relevant for the analysis.
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