An apparatus for determining a coordinate transformation for mixing an image of a first subject into an X-ray image of a second subject has an arrangement for fastening the apparatus to the second subject, markers that can be acquired by a navigation system, and X-ray-positive marks. The positions and orientations of the markers that can be acquired with the navigation system and the positions and orientations of the X-ray-positive marks relative to one another are thus known, so a coordinate transformation can be determined between a coordinate system allocated to the navigation system and a coordinate system allocated to the X-ray image. Thus an image of the first subject that can be acquired with the navigation system can be mixed into an X-ray image of the second subject.