A method is described for separating, retrieving and concentrating platelets
from
whole blood relying on aggregation of the platelets followed by filtration. This
method eliminates the need of a centrifuge for separating said cells from blood.
To obtain cellular concentrates of platelets, blood is mixed with compatible agents
that will aggregate cells while retaining contained growth factors. The resulting
aggregates can then be separated from blood by filtration. If desired, the filter-captured
aggregates are subject to a brief washing cycle where they are washed clean of
residual aggregating agent, plasma, and red cells. Aggregates can then be partly
or wholly deaggregated and the cells retrieved. The result is a suspension of cells
and small aggregates with therapeutic levels of concentrated blood cells with included
growth factors that are available for delivery to a wound site. A device that accomplishes
the aforementioned process is also described.