A process for separation of components of a sample in an ion mobility spectrometer using a supply of selectively interactive gaseous particles. The components to be separated carry out selective interaction processes in a drift tube of the ion mobility spectrometer with specifically selected particles, in particular enantiometric collision molecules, which are supplied to a gas inlet, in such a way that they exhibit different rates of mobility during their drift from a sample inlet through the drift tube to an ion collector. This enables the ions to be detected in the ion collector in a time-delayed manner.