A personal hydration system is provided including a pre-filled reservoir pouch. The pouch may be filled with a drink fluid by a supplier, heated, and aseptically sealed. The pouch may also be cold-filled with a drink fluid and preservatives and then sealed. The pouch is preferably designed as a disposable single-use item. In order to keep the pouch contents fresh and to facilitate delivery of the fluid from the reservoir through a drink tube, the pouch includes a unique tube connector structure. A first connector portion is mounted to a wall of the pouch. The first connector portion includes a closure member that keeps fluid sealed in the bladder. A second connector portion is matable with the first connector portion and includes a structure that operates to move the closure member to an open position when mated with the first connector portion. The second connector portion is shaped to fit with a conventional flexible drinking tube.