Disclosed are processes for producing stably transformed fertile wheat
a system of transforming wheat via Agrobacterium. This invention provides
methods transforming a variety of explants, such as freshly isolated or pre-cultured
immature embryos, embryogenic callus and suspension cells. Also disclosed are methods
for recovering transgenic plants after transformation within a short period of
time, if the explants are regenerable at the time of transformation. Thus the frequency
of somaclonal variation associated with prolonged in vitro culture period is significantly
reduced. The transformation frequency using this system is comparable to or better
than published methods using other systems, such as microprojectile bombardment.