A urine collection assembly includes a urine collection device and a urine collection chamber in communication with the urine collection device. The urine collection chamber includes a collection reservoir, an entrance conduit having an inlet to receive urine from the urine collection device and an outlet terminating in the reservoir, an anti-reflux valve, and an exit conduit. The collection reservoir is configured to be wearable in close proximity to a user's body below the waist and above the knees of the user. The collection reservoir holds urine excreted from a user. The exit conduit enables drainage of the collection reservoir. The exit conduit also enables attachment of the collection reservoir to an additional conventional urine collection chamber. The anti-reflex valve is configured to prevent urine from backing up out of the urine collection chamber and into the urine collection device. The inlet of the urine collection chamber is disposed in close approximation to the urine collection device to direct urine from the urine collection device to the collection reservoir. The urine collection assembly further can include a support device wearable by the user to bear the weight of the urine collection chamber and to support the inlet of the urine collection chamber in close approximation to the urine collection device.