The present invention provides a method of producing a plant which
exhibits improved growth and/or yield under reduced nitrogen conditions,
that is, under cultivation conditions where nitrogen is limited as
compared to ordinary cultivation conditions, by increasing 2-OG content
in plants. Particularly, the present invention provides a method of
producing a plant which exhibits improved growth and/or yield under
conditions where nitrogen is reduced, that is, under cultivation
conditions where nitrogen is limited as compared to ordinary cultivation
conditions, by introducing a GDH gene or ECASPC gene into plants and
expressing the transgene GDH or ECAPS in the plants, which results in
increased 2-OG, or by spraying proline on the leaves of plants to
increase the 2-OG content, thereby enhancing the incorporation of
nitrogen or metabolic activity of plants. Also provided is a method of
cultivating such plants under nitrogen-limited conditions.