The take-off needle comprises a main hollow lance having a distal end adapted to perform a pricking operation, said hollow lance being connected to two pipes each provided with a spigot for it to be connected to a tube, said needle further comprising a body for holding the main hollow lance, said holding body defining two internal cylindrical surfaces forming said pipes, the main hollow lance comprising, at its proximal end, a sleeve having an internal channel of which the distal end is connected to the proximal end of the main hollow lance, and of which the proximal end opens into the holding body, said sleeve comprising at its proximal end two crescent-shaped surfaces inclined towards the interior of the internal channel and towards the distal end of the sleeve, each of said crescent-shaped surfaces being disposed so as to extend in line with one of the internal cylindrical surfaces of the holding body.

 
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> Chromatographic column seal

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