The present invention claims and discloses a novel apparatus and method for efficiently
cultivating cells with minimal mortality in order to harvest a maximum amount of
cellular products generated by the cultivated cells. More particularly, the present
invention teaches a method and a device for plating cells and causing maximum adherence
of cells of interest. Furthermore, the present invention also teaches a growth
substrate means that is capable of providing the largest surface area for cell
adhesion and functions as an oxygenator, a depth filter and a static mixer to maximize
the production of cellular products by intermittently and periodically provide
sufficient oxygen and nutrients to the cells without causing cell death. The device
of the present invention is economical and can be disposable thus eliminating complications
caused by sterlization and is capable of periodically and intermittently provide
oxygen and nutrients to cells, through controlling the amount of culture medium
that comes into contact with the growth substrate means.