An insect holder for holding an insect, e.g. a forager honey bee, comprises a housing with a chamber adapted to receive the insect, an inlet to the chamber through which the insect can pass to enter the chamber, and a head opening to the chamber adapted to permit the head of the insect in the chamber to pass therethrough to the exterior of the housing while retaining the insect in the housing, with the head of the insect protruding through the head opening to the exterior of the housing. The insect is constrained to be unable to turn around in the chamber or withdraw its head into the chamber, so that observations can be made on the insect head, e.g. monitoring proboscis movement in known manner. When observations are finished, the insect is released unharmed and undamaged.