The present invention relates to nematode-regulated promoter sequences and their use in creating or enhancing nematode-resistance in plants. Nucleic acid molecules comprising a heterologous nucleotide sequence operably linked to a nematode-regulated promoter and vectors, plant cells, plants, and transformed seeds containing such constructs are provided. Methods for the creation and use of such promoters in repressing or inducing expression of a heterologous nucleotide sequence in a plant, as well as methods for creating or enhancing nematode-resistance in plants by such repression or induction of heterologous nucleotide sequences by nematode-regulated promoters are also provided.