A donor substrate for use in an organic light emitting display comprises a base substrate and a transfer layer disposed on the base substrate. A selective heat generation structure is interposed between the base substrate and the transfer layer. The selective heat generation structure has a heat generation region from which heat is generated by light-to-heat conversion and a heat non-generation region contacting the heat generation region. By employing the donor substrate, it is possible to form minute transfer layer patterns with high accuracy without the need to accurately control the width of a laser beam. A fabrication method of an organic light emitting display comprises disposing the donor substrate on an acceptor substrate, irradiating a laser beam onto the donor substrate, and forming a transfer layer pattern on a pixel electrode of the acceptor substrate.