The present invention relates to a milk-coagulating enzyme of bacterial
origin and a process for producing cheese by using this enzyme, namely,
an enzyme that is produced by a bacterium belonging to the genus
Paenibacillus and exhibits milk-coagulating activity, characterized by
having the following enzymological properties: 1) function: having an
activity of coagulating milk to form a curd; 2) substrate specificity:
acting on .kappa.-casein as a substrate and specifically cleaving
Thr94-Met95 in the presence of calcium; 3) optimum pH: 6.0-7.0, and 4)
molecular weight: 35,000-37,000 Da when measured by SDS-PAGE; a process
for producing the enzyme; a process for producing cheese; and a
cheese-like food product.