The invention relates to a method for preparing free polyunsaturated fatty acids and their oxidation metabolites, wherein, successively,

    • the production of polyunsaturated fatty acids and of their oxidation metabolites is stimulated, in a red alga, by the action of an elicitor which is peptide, lipid or saccharide in nature, and then
    • the polyunsaturated fatty acids produced and also their oxidation metabolites are extracted.

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