A method andsystem for guiding and positioning a self-propelled vehicle with sequential barcodes has a self-propelled vehicle capable of moving on the strip holder according to the recorded barcode serial numbers on the strip holder. Furthermore, due to the barcode serial number is sequential and the gaps between the barcodes are the same, the self-propelled vehicle can calculate its velocity, distance of travel and a distance to a target location according to the barcode serial numbers. Moreover, the self-propelled vehicle can select different important positioning points.